Centromere protein B

Protein-coding gene in the species Homo sapiens

CENPB

Available structures

PDB

Ortholog search: PDBe RCSB

List of PDB id codes
1UFI, 1BW6, 1HLV

Identifiers

Aliases

CENPB, entrez:1059, centromere protein B

External IDs

OMIM: 117140 MGI: 88376 HomoloGene: 1370 GeneCards: CENPB

Gene location (Human)

Chr.	Chromosome 20 (human)^[1]

Band	20p13	Start	3,783,851 bp^[1]
Band	20p13	End	3,786,740 bp^[1]

Gene location (Mouse)

Chr.	Chromosome 2 (mouse)^[2]

Band	2 F1\|2 63.29 cM	Start	131,017,102 bp^[2]
Band	2 F1\|2 63.29 cM	End	131,021,987 bp^[2]

RNA expression pattern

Bgee

Human

Mouse (ortholog)

Top expressed in

amygdala

gastrocnemius muscle

putamen

stromal cell of endometrium

nipple

inferior ganglion of vagus nerve

nucleus accumbens

caudate nucleus

right coronary artery

hypothalamus

Top expressed in

parotid gland

superior frontal gyrus

lacrimal gland

extensor digitorum longus muscle

intercostal muscle

brown adipose tissue

olfactory epithelium

right lung

soleus muscle

More reference expression data

BioGPS

n/a

Orthologs

Species

Human

Mouse

Entrez


1059


12616

Ensembl


ENSG00000125817


ENSMUSG00000068267

UniProt


P07199


P27790

RefSeq (mRNA)


NM_001810


NM_007682

RefSeq (protein)


NP_001801


NP_031708

Location (UCSC)

Chr 20: 3.78 – 3.79 Mb

Chr 2: 131.02 – 131.02 Mb

PubMed search

^[3]

^[4]

Wikidata

View/Edit Human

View/Edit Mouse

Centromere protein B also known as major centromere autoantigen B is an autoantigen protein of the cell nucleus. In humans, centromere protein B is encoded by the CENPB gene.^[5]^[6]^[7]

Function

Centromere protein B is a highly conserved protein that facilitates centromere formation. It is a DNA-binding protein that is derived from transposases of the pogo DNA transposon family. It contains a helix-loop-helix DNA binding motif at the N-terminus and a dimerization domain at the C-terminus. The DNA binding domain recognizes and binds a 17-bp sequence (CENP-B box) in the centromeric alpha satellite DNA. This protein is proposed to play an important role in the assembly of specific centromere structures in interphase nuclei and on mitotic chromosomes. It is also considered a major centromere autoantigen recognized by sera from patients with anti-centromere antibodies.

Clinical significance

Centromere protein B is a potential biomarker of small-cell lung cancer.^[8]

References

^ ^a ^b ^c GRCh38: Ensembl release 89: ENSG00000125817 – Ensembl, May 2017
^ ^a ^b ^c GRCm38: Ensembl release 89: ENSMUSG00000068267 – Ensembl, May 2017
^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ "Entrez Gene: centromere protein B".
^ Sugimoto K, Yata H, Himeno M (July 1993). "Mapping of the human CENP-B gene to chromosome 20 and the CENP-C gene to chromosome 12 by a rapid cycle DNA amplification procedure". Genomics. 17 (1): 240–2. doi:10.1006/geno.1993.1312. PMID 8406460.
^ Ando S, Yang H, Nozaki N, Okazaki T, Yoda K (April 2002). "CENP-A, -B, and -C chromatin complex that contains the I-type alpha-satellite array constitutes the prekinetochore in HeLa cells". Molecular and Cellular Biology. 22 (7): 2229–41. doi:10.1128/MCB.22.7.2229-2241.2002. PMC 133672. PMID 11884609.
^ Briasoulis E, Kamposioras K, Tzovaras V, Pafitanis G, Kostoula A, Mavridis A, Pavlidis N (May 2008). "CENP-B specific anti-centromere autoantibodies heralding small-cell lung cancer. A case study and review of the literature". Lung Cancer. 60 (2): 302–6. doi:10.1016/j.lungcan.2007.09.014. PMID 17980453.

Saxena A, Wong LH, Kalitsis P, Earle E, Shaffer LG, Choo KH (September 2002). "Poly(ADP-ribose) polymerase 2 localizes to mammalian active centromeres and interacts with PARP-1, Cenpa, Cenpb and Bub3, but not Cenpc". Human Molecular Genetics. 11 (19): 2319–29. doi:10.1093/hmg/11.19.2319. PMID 12217960.
Nousiainen M, Silljé HH, Sauer G, Nigg EA, Körner R (April 2006). "Phosphoproteome analysis of the human mitotic spindle". Proceedings of the National Academy of Sciences of the United States of America. 103 (14): 5391–6. Bibcode:2006PNAS..103.5391N. doi:10.1073/pnas.0507066103. PMC 1459365. PMID 16565220.
Orthaus S, Biskup C, Hoffmann B, Hoischen C, Ohndorf S, Benndorf K, Diekmann S (January 2008). "Assembly of the inner kinetochore proteins CENP-A and CENP-B in living human cells". ChemBioChem. 9 (1): 77–92. doi:10.1002/cbic.200700358. PMID 18072184. S2CID 37467765.
Tawaramoto MS, Park SY, Tanaka Y, Nureki O, Kurumizaka H, Yokoyama S (December 2003). "Crystal structure of the human centromere protein B (CENP-B) dimerization domain at 1.65-A resolution". The Journal of Biological Chemistry. 278 (51): 51454–61. doi:10.1074/jbc.M310388200. PMID 14522975.
Tanaka Y, Tachiwana H, Yoda K, Masumoto H, Okazaki T, Kurumizaka H, Yokoyama S (December 2005). "Human centromere protein B induces translational positioning of nucleosomes on alpha-satellite sequences". The Journal of Biological Chemistry. 280 (50): 41609–18. doi:10.1074/jbc.M509666200. PMID 16183641.
Wellcome Trust Case Control Consortium; et al. (June 2007). "Genome-wide association study of 14,000 cases of seven common diseases and 3,000 shared controls". Nature. 447 (7145): 661–78. Bibcode:2007Natur.447..661B. doi:10.1038/nature05911. PMC 2719288. PMID 17554300.
Tanaka Y, Kurumizaka H, Yokoyama S (January 2005). "CpG methylation of the CENP-B box reduces human CENP-B binding". The FEBS Journal. 272 (1): 282–9. doi:10.1111/j.1432-1033.2004.04406.x. PMID 15634350. S2CID 45738717.
Sowa ME, Bennett EJ, Gygi SP, Harper JW (July 2009). "Defining the human deubiquitinating enzyme interaction landscape". Cell. 138 (2): 389–403. doi:10.1016/j.cell.2009.04.042. PMC 2716422. PMID 19615732.
Lim J, Hao T, Shaw C, Patel AJ, Szabó G, Rual JF, Fisk CJ, Li N, Smolyar A, Hill DE, Barabási AL, Vidal M, Zoghbi HY (May 2006). "A protein-protein interaction network for human inherited ataxias and disorders of Purkinje cell degeneration". Cell. 125 (4): 801–14. doi:10.1016/j.cell.2006.03.032. PMID 16713569. S2CID 13709685.
Lomonte P, Morency E (February 2007). "Centromeric protein CENP-B proteasomal degradation induced by the viral protein ICP0". FEBS Letters. 581 (4): 658–62. doi:10.1016/j.febslet.2007.01.027. PMID 17258208. S2CID 27748450.
Suzuki N, Nakano M, Nozaki N, Egashira S, Okazaki T, Masumoto H (February 2004). "CENP-B interacts with CENP-C domains containing Mif2 regions responsible for centromere localization". The Journal of Biological Chemistry. 279 (7): 5934–46. doi:10.1074/jbc.M306477200. PMID 14612452.
Hellwig D, Münch S, Orthaus S, Hoischen C, Hemmerich P, Diekmann S (August 2008). "Live-cell imaging reveals sustained centromere binding of CENP-T via CENP-A and CENP-B". Journal of Biophotonics. 1 (3): 245–54. doi:10.1002/jbio.200810014. PMID 19412974. S2CID 22855465.
Robitaille G, Hénault J, Christin MS, Senécal JL, Raymond Y (November 2007). "The nuclear autoantigen CENP-B displays cytokine-like activities toward vascular smooth muscle cells". Arthritis and Rheumatism. 56 (11): 3814–26. doi:10.1002/art.22972. PMID 17968937.
Obuse C, Yang H, Nozaki N, Goto S, Okazaki T, Yoda K (February 2004). "Proteomics analysis of the centromere complex from HeLa interphase cells: UV-damaged DNA binding protein 1 (DDB-1) is a component of the CEN-complex, while BMI-1 is transiently co-localized with the centromeric region in interphase". Genes to Cells. 9 (2): 105–20. doi:10.1111/j.1365-2443.2004.00705.x. PMID 15009096. S2CID 21813024.
Perdoni F, Bottone MG, Soldani C, Veneroni P, Alpini C, Pellicciari C, Scovassi AI (August 2009). "Distribution of centromeric proteins and PARP-1 during mitosis and apoptosis". Annals of the New York Academy of Sciences. 1171 (1): 32–7. Bibcode:2009NYASA1171...32P. doi:10.1111/j.1749-6632.2009.04729.x. PMID 19723035. S2CID 42584743.
Andersen JS, Lam YW, Leung AK, Ong SE, Lyon CE, Lamond AI, Mann M (January 2005). "Nucleolar proteome dynamics". Nature. 433 (7021): 77–83. Bibcode:2005Natur.433...77A. doi:10.1038/nature03207. PMID 15635413. S2CID 4344740.
Robitaille G, Christin MS, Clément I, Senécal JL, Raymond Y (September 2009). "Nuclear autoantigen CENP-B transactivation of the epidermal growth factor receptor via chemokine receptor 3 in vascular smooth muscle cells". Arthritis and Rheumatism. 60 (9): 2805–16. doi:10.1002/art.24765. PMID 19714638.